Cancer immunotherapy is increasingly being evaluated as an approach to cancer treatment by harnessing the immune system to attack cancer cells. Both the adaptive and innate arms of the immune system ...

Flow cytometry assay validation requires different analytical approaches in preclinical and clinical settings. A fit-for-purpose (FFP) strategy allows researchers to customize the validation to ...

T naive cells (T N), T central memory cells (T CM), T stem cell-like memory cells (T SCM), T effector memory cells (T EM), T transitional memory cells (T TM), T effector memory cell re-expressing ...

The Agilent NovoCyte Quanteon flow cytometer and xCELLigence Real-Time Cell Analysis platform were used to develop a series of in vitro preclinical CAR T-cell safety and efficacy assays Cancer ...

This handbook is designed as a go-to reference for every flow cytometry user. Scientists using traditional systems will learn how routine workflows can benefit from the speed, throughput, and ...

This article is based on a poster originally authored by Sophie Snow, Kira Freeman, Zaynab Isseljee, Alice Brankin and Christine Sanfelice, which was presented at ELRIG Drug Discovery 2024 in ...

One of the primary objectives for the application of flow cytometry in any testing environment should be measurement assurance, i.e., the generation of reliable and reproducible results. This goal can ...

Human umbilical vein endothelial cells (HUVECs) were seeded at 1 × 10 4 cells per cm 2 in six-well plates. Treatment with TNF-α (2.5, 5 or 10 ng/ml) or lipopolysaccharide (0.5, 1 or 5 μg/ml) treatment ...

How do we “name” a cell and assign its identity? How do we know that we are all talking about the same cells? How do we agree on what it takes to confidently correlate previous with current research ...

Flow cytometry is an invaluable method for biomedical research. Since its development over 50 years ago, technology for flow cytometry has progressed rapidly, allowing for the detection of more and ...